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. Author manuscript; available in PMC: 2016 Apr 17.
Published in final edited form as: J Immunol. 2014 Dec 15;194(3):1090–1099. doi: 10.4049/jimmunol.1402401

FIGURE 4.

FIGURE 4

α-Toc supplementation abolishes pulmonary inflammation in aged mice after S. pneumoniae infection. Young and aged mice fed a control α-Toc 30 (white bars) or α-Toc–supplemented (α-Toc 500; black bars) diet were infected intratracheally with 104 S. pneumoniae and inflammatory cytokine levels in lung homogenates (A) as well as the total number of PMNs (B) (Ly6G+) in the lungs were compared 48 h postinfection. Pooled data from four separate experiments (n = 12 per group) are shown, and were analyzed by two-way ANOVA followed by Kruskal–Wallis test. Groups marked “a” are significantly (p < 0.001) different from those marked “b,” whereas groups marked “b” are statistically indistinguishable from each other. (C) The number of PMNs in the bronchoalveolar lavage fluid of aged mice was also enumerated. (D) The lungs were also sectioned stained with H&E and examined by light microscopy. Original magnification ×10. Pooled data from two separate experiments (n = 6 per group) are shown. *p < 0.05 indicates means are significantly different by Mann–Whitney U test.