FIGURE 7.

α-Toc has no effect on 12-lipoxygenase levels or activity. Epithelial cells were treated overnight with vehicle control (Veh. cntrl; white bars) or α-Toc (black bars) at 100 µg/ml or the indicated concentrations. (A) 12- and 15-lipoxygenase protein levels were then assessed by intracellular staining followed by flow analysis of H292 cells treated with S. pneumoniae or left uninfected. Representative from one of four separate experiments, in which each condition was tested in triplicate per experiment, are shown. *p < 0.05 indicates means significantly different by unpaired Student t test. (B) The amount of the metabolite 12(S)-HETES released into the supernatants was measured by ELISA. **p < 0.001 by unpaired Student t test. Representative data from one of three separate experiments, in which each condition was tested in triplicate per experiment, are shown. (C) PMN migration in response to HXA₃ (200 ng/ml) added to the lower chamber (apically) was measured by MPO ELISA. Representative data from one of two separate experiments, in which each condition was tested in triplicate per experiment, are shown. *p < 0.05, ***p < 0.0001 indicates means significantly different from vehicle control by unpaired Student t test.