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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Exp Neurol. 2016 Feb 15;279:13–26. doi: 10.1016/j.expneurol.2016.02.009

Figure 8. The effects of 4PBA and VX563 on histone deacetylase (HDAC) activity in SMNΔ7 SMA mice.

Figure 8

HDAC activity was measured by the levels of acetylated histone H3 (H3) in spinal cord extracts. SMNΔ7 SMA mice (n = 3/group) were treated with either 4PBA, VX563 or their appropriate vehicles for 5 days beginning at PND04. Age-matched non-SMA mice were also included as controls. The effects of these compounds on both the levels of and the acetylation of H3 were determined by immunoblot (A). The band intensities for either H3 or acetylated H3 (Ac-H3) were normalized against those for the loading control β-actin. The levels of H3 protein (B) were not affected by drug but tended to be lower in SMNΔ7 SMA spinal cord samples. These differences were not statistically significant. The acetylation of H3 at lysine 9 (K9) (C) was greater in spinal cord samples from SMNΔ7 SMA mice treated with 4PBA or VX563. The differences, however, were not statistically significant due to Ac-H3 variability within treatment groups.