Figure 3.

Suppression of oscillatory mitochondrial Ca²⁺ signals in a spontaneously pacing control hiPSC-CM infected with the genetically engineered targeted, Ca²⁺ probe, myticam-E31Q [26]. (A) Tracings showing changes in regional mitochondrial Ca²⁺ signals (top) and I_NCX (bottom) before and 30 s after exposure to 50 nM FCCP. Increasing mitochondrial Ca²⁺ corresponds to downward deflection. (B) Images show baseline fluorescence (F₀) and color-coded regions of interest (ROI, with “n” indicating nuclei) and 4 differential fluorescence images (measured at the times indicated along the traces) showing repeatable mitochondrial Ca²⁺ signals before FCCP application (1, 2, 3) and smaller, more localized responses after (4). Modified from [45]