Table 1.
Calcium signaling characteristic of various hiPSC-CM cell lines.
| hiPSC-CM Cell-lines | mRNA | Immunostaining | Action Potential | ICa | SR Ca2+ content | INCX | SERCA | Spontaneous Ca2+ transients | Ca2+ sparks | IP3R |
|---|---|---|---|---|---|---|---|---|---|---|
| [69] | NKX2.5 MLC2v ACTN2 MYH6 GATA4 a |
No data | No data | No data | ++ | No data | No data | ++ | No data | No data |
| [40] | RyR2, IP3R2, SERCA2a Cav1.2 PLB; CSQ |
α-actinin & RyR2 | No data | No data | ++++ | No data | Thapsigargin suppress spontaneous and caffeine induced Ca2+ transients | suppressed by nifedipine or ryanodine | No data | 2-APB or U73122 suppress Ca2+ transients |
| [28] | NCX1, RyR2, SERCA2a, MHC | α-actinin & TPM | 45% N-CM, 38% V-CM, 17% A-CM | No data | + | No data | No data | U-shape Ca2+ wave; rise of calcium in the periphery faster than center | No data | No data |
| [41] | No data | RyR2 & CSQ | No data | No data | + | No data | No data | ++ | No data | No data |
| [35] | No data | No data | 54% V-CM, 22% N-CM, 24% A-CM | ICa, bell shape I-V | No data | No data | No data | No data | No data | No data |
| [33] [45] | No data | RyR2 | No data | ICa, bell shape I-V | ++++ | ++++ | Thapsigargin suppress spontaneous Ca-transients | Suppressed by NCX or RyR2 blockers | Sporadic and brief | 2-APB or U73122 slightly affect spontaneous beating |
| [29] | No data | α-actinin & α-MHC | 61% V-CM, 17.4% A-CM and 21.6% N-CM, | No data | No data | No data | No data | Ca2+ increase first at periphery then propagate to centre | Stochastic and repetitive | No data |
| [34] | No data | No data | No data | ICa bell shape I-V | ++++ | ++++ | SERCA Ca2+ transport are functional | Slower time to peak and decay rates | No data | No data |
CSQ: calsequestrin; PLB Phospholamban; MHC: Myosin heavy chain; RyR2: Ryanodine receptor type 2; NCX: Sodium Calcium exchanger; TPM Tropomyosin