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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Hepatol. 2016 Jan 19;64(5):1137–1146. doi: 10.1016/j.jhep.2016.01.010

Figure 4. Separation of HSCs, PFs, and MCs from the Col1a1GFP livers by FACS.

Figure 4

(A) NPCs prepared from wild-type or Col1a1GFP livers were stained with PI and anti-GPM6A antibodies. PI− cells were analyzed for autofluorescence of VitA and VitA+ HSCs were sorted (P1). The VitA− fraction was further analyzed for the expression of GPM6A and GFP into P2–P4 factions. Wild-type or Col1a1GFP NPCs incubated with the isotype IgG were used as negative controls. (B) QPCR of cells before FACS (All) and P1–P4 fractions obtained by FACS. *P<0.05, **P<0.01.