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. 2016 Apr 18;7:134. doi: 10.3389/fimmu.2016.00134

Figure 5.

Figure 5

KIR3DL1 expression on CD8+ T-cells is required for HLA-B:Bw4-80Ile-dependent viral suppression. (A) Cytometric analysis of KIR expression in CD8+ T-cells freshly taken from a representative EC (No. 3). (B) Pan-KIR (total inhibitory KIRs) and KIR3DL1-expressing CD8+ T-cells were significantly higher in ECs (No. 1–5) (green) than in HVLpts carrying KIR3DL1 gene (No. 2, 3, and 6–8). (C) CD8+ T-cell-mediated viral suppression was reduced by 4–5 logs when freshly pan-KIR or KIR3DL1-depleted CD8+ T-cells were added as compared with bulk CD8+ T-cells. (D) CD8+ T-cell-mediated viral suppression was reduced by 3–4 logs in Bw4-80I/KIR3DL1-matched patients (EC#1–5) when the anti-KIR3DL1 antibody (clone Dx9) was added as compared with parallel cultures in the presence of anti-KIR3DS1 antibody (clone z27) or medium alone. In contrast, the low levels of viral suppression observed in Bw4-80I/KIR3DL1-mismatched patients (EC#6, HVLpt#2, and HVLpt#4) remained unaffected by the anti-KIR3DL1 antibody (clone Dx9).