Figure 2.

Glucose deprivation increases astroglial volume. (A) Sample confocal images of an enhanced green fluorescent protein (eGFP) labeled hippocampal astrocyte from a glial fibrillary acidic protein (GFAP)-eGFP mouse illustrated before (Control) and after 30 min of exogenous glucose deprivation. Scale bar, 10 μm. (B) Time-dependent changes in astrocytic total (red circles), soma (green circles) and processes (blue triangles) volumes were quantified using 3D confocal morphometry analysis in each individual cell every 5 min during exogenous glucose deprivation and after 10 min of washout. Volume changes were normalized to values measured at t = 0 and expressed relative to this baseline as an increase in percentage. Glucose deprivation increased significantly all astrocytic volumes, and this effect was partially reversible after 10 min of glucose re-introduction (n = 22 cells, 10 slices, 4 mice). Asterisks indicate statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001).