Table 1.

Primers used for the amplification of mitochondrial- and nuclear target genes in order to quantify host mitochondrial genome copy number (mtDNA/nucDNA ratio) in Exaiptasia pallida.

Objective	Target	Size (bp)	Forward Primer (5′–3′)	Reverse Primer (3′–5′)
TOPO® Cloning (standard curve)	COI	356	AGC CGT CAG AGA CAG TAA	CGT GAC CAA GCC CTA ATA AA
	ATP6	342	GGT TGT TAC GTT AGG CTT GT	ACT TGA ATA ACC GCC ACT
	EF-1-α	803	TTC TCT CTT ACA CCC TTG G	CTT GTC AGT GGG TCT CTT
QPCR	COI	101	GTC TCC CAG CCG GAA ATA AA	ACC ATT GTC AGC ATC TCT CG
	ATP6	144	CGT CTC GCC GCA AAT TTA TC	TGC GGC CTC TAG TAG ACT TA
	EF-1-α	89	AGC ACT GAG CCA CCA TAC AG	TTG GGT TAT AGC CGG TCT TC

CO1, Cytochrome c oxidase subunit 1; ATP6, ATP synthase subunit 6; EF-1-α, Eukaryotic translation elongation factor 1-alpha. Annealing temperature was 60°C for all primer sets. Specific details of PCR conditions are provided in the accompanying Electronic Supplementary Information.