Figure 6.

PGE₂ mediates the HLSC activity on monocyte-derived DCs. (a) Cell supernatants were harvested to detect PGE₂ production by ELISA after 3 and 7 days of coculture of DCs in the presence or the absence of HLSCs. ANOVA with Dunnet's multicomparison test was performed: ^∗∗ p < 0.001 DC+HLSC versus HLSC. (b) mRNA expression levels of Cox-1 and Cox-2 were evaluated in HLSCs and in DCs cocultured for 7 days. Cox-1 and Cox-2 expression levels were higher in HLSCs compared with DCs. Data are expressed as relative quantification (RQ) using ΔΔCt method. Normalization was made using actin as housekeeping gene. ANOVA with Dunnet's multicomparison test was performed: ^∗∗ p < 0.001 HLSC versus DC. (c) Mean of percentages ± SD of CD14, CD80, CD86, CD1a, CD54, and α4 and α5 integrin of positive DCs differentiated in the presence (HLSC) or in the absence (CTRL) of HLSCs or stimulated with NS-398. Results were obtained from 4 independent experiments. ANOVA with Dunnet's multicomparison test was performed: ^∗ p < 0.05 HLSC versus HLSC+NS-398; ^∗∗ p < 0.001 HLSC versus HLSC+NS-398.