FIGURE 4.

Treatment with IFN-γ can overcome deficient NO production in DBA/2 BMDMs to control M. tuberculosis growth. BMDMs were infected with virulent H37Rv or attenuated H37Ra for 48 or 72 h. (A) The levels of NO that were secreted into the supernatants were measured. The data are presented as the mean ± standard deviation of experiments performed in triplicate (left panel). iNOS levels were measured in the BMDMs using Western blot assays (right panel). (B,C) BMDMs were stimulated with IFN-γ (20 ng/ml) after infection with H37Rv or H37Ra. (B) The level of NO that was secreted into the supernatants was measured using an NO detection kit. The data are presented as the mean ± standard deviation of experiments that were performed in triplicate (left). iNOS levels were determined in the BMDMs using Western blot assays (right). (C) Bacterial growth was confirmed using CFU assessments. The data are presented as the mean ± standard deviation of triplicate experiments. All of the experiments were independently repeated three to five times for each condition with similar results. Significant differences are indicated by ^∗∗∗P < 0.001.