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. 2016 Apr 18;6:24434. doi: 10.1038/srep24434

Figure 2. JNK1/2 signaling plays the major role in the MAPK-mediated apoptosis in Jurkat T cells by anisomycin.

Figure 2

(A–F) Jurkat T cells were treated with the increasing concentrations of anisomycin for 1 h or with 40 ng/ml of anisomycin at the indicated time. Following the treatment, the expressions of ERK1/2, P-ERK1/2 (A,B), p38, P-p38 (C,D), JNK1/2 and P-JNK1/2 (E,F) proteins were determined by Western blotting. (G,H) The cells were pretreated with 10 μM SP600125 (SP), 20 μM PD98059 (PD), 20 μM SB203580 (SB) or the increasing concentrations of SP600125 before the exposure to 40 ng/ml of anisomycin. Then, the expressions of JNK1/2 and P-JNK1/2 were examined by Western blotting. (I,J) Simultaneously, the apoptotic phenotypes of the cells treated above were observed using annexin-V/PI double staining under the flow cytometer. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, ** P < 0.01 vs. the untreated control, #P < 0.05, ##P < 0.01 vs. the 40 ng/ml anisomycin group.