Table 4.

Immunoreactive Peptide Levels in Stabilized Tissue After Different Extraction Procedures

Test procedure	DYNB	LARG	MEAP
A. Hypothalamus
1  1 M acetic acid, 2 × 95°C	34.8 ± 3.92	ND	433 ± 21.4
2  1 M acetic acid, 1 × 95°C	34.0 ± 3.34	ND	386 ± 20.3
3  1 M acetic acid	42.1 ± 2.21	ND	436 ± 22.9
4  0.25% acetic acid	34.5 ± 2.14	ND	471 ± 16.1
5  Methanol/water/acetic acid	33.1 ± 1.57	ND	489 ± 34.0
B. Forebrain
1  1 M acetic acid, 2 × 95°C	11.8 ± 0.34	1.70 ± 0.04	128 ± 2.41
2  1 M acetic acid, 1 × 95°C	10.9 ± 0.29	1.40 ± 0.08*	131 ± 4.84
3  1 M acetic acid	12.0 ± 0.32	1.37 ± 0.05**	108 ± 2.60**
4  0.25% acetic acid	9.78 ± 0.20**,+++	1.18 ± 0.04***,+	111 ± 2.76**
5  Methanol/water/acetic acid	9.21 ± 0.28***,+++	1.19 ± 0.07***	129 ± 7.24

The ir peptide levels were measured in pooled stabilized hypothalamus (A) and forebrain (B), respectively, after different extraction procedures according to Figure 2.

^*p < 0.05; ^**p < 0.01; ^***p < 0.001 compared to test procedure 1 (standard procedure).

⁺p < 0.05; ⁺⁺⁺p < 0.001 compared to test procedure 3.

ir, immunoreactive.