FIGURE 6.

Adoptive transfer of splenocytes into Rag^−/− mice. Ten million splenocytes from WT C57BL/6 mice were transferred into Rag1^−/− mice. Spleens were harvested 14 d later. Multiplex fluorescent IHC was performed on Rag^−/− mice (A–E) and adoptively transferred animals (F–J). (K–N) The percentage of positive cells was enumerated based on total DAPI⁺ cells in the area imaged. The p values were calculated using the unpaired t test (n = 3–4). Original magnification ×20.