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. 2016 Apr 18;6:24381. doi: 10.1038/srep24381

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(a) Phase-contrast microscopy of a co-culture of B. bacteriovorus HD100 preying on P. putida KT2440 at the onset of predation (time zero) and (b) after 40 h of incubation. (c,d) 1:100 dilution of the co-cultures from panels (a,b), respectively. Mcl-PHA granules can be observed in the extracellular medium after 40 h of predation. (e) Cell viability assay of the co-culture of B. bacteriovorus HD100/P. putida KT2440 (white bars and black bars, respectively) at the onset of predation (time zero) and within 40 h. (f) Total PHA content in the co-culture of B. bacteriovorus HD100/P. putida KT2440 compared to the control culture (KT2440 without the predator) at the onset of predation (time zero) (white bars) and within 40 h (black bars). Asterisks (^*) indicate significant differences (^*P < 0.05).