Figure 5. Mcl-PHA hydrolytic product profile identified in the co-culture supernatants of B. bacteriovorus strains preying on P. putida KT2440 and KT42Z accumulating mcl-PHA.
(a) HPLC-MS analysis after 30 h of predation by Bdellovibrio strains. Monomers (white bars), dimers (light grey bars) and trimers (dark grey bars). Control supernatants of P. putida KT2440 and KT42Z are also shown. (b) Total PHA hydrolysis products quantified in the culture supernatants. No significant differences were observed between: (i) the percentage of monomers of the PHA extracted from KT2440 and Bd3709/KT2440, (ii) the percentage of dimers of KT2440 and HD100/KT2440, (iii) the percentage of dimers of KT2440 and Bd3709/KT2440, and (iv) the percentage of dimers of KT2440 and dimers of Bd3709/KT42Z. The rest of the conditions showed significant differences (P < 0.05) determined by ANOVA-test.