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. 2016 Apr 18;6:23476. doi: 10.1038/srep23476

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Copyright © 2016, Macmillan Publishers Limited

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(a) Relative expression of Mknk1 (MNK1) and Mknk2 (MNK2) was determined by qPCR in liver and gonadal adipose tissue (n = 8). Data are mean ± SEM. (b) Immunoblot analysis of MNK1 protein levels in liver, adipose tissue, heart and skeletal muscle; representative of 4 independent experiments. (c) mRNA expression of PPARγ, C/EBPα, SREBP1c, GLUT4 and CD36 in differentiating 3T3-L1 adipocytes relative to day 0. Data are mean ± SEM, one-way ANOVA with Tukey’s post-test (n = 3). (d) mRNA expression for Mknk1 (MNK1) and Mknk2 (MNK2) in differentiating 3T3-L1 adipocytes relative to day 0 (n = 3). Data are mean ± SEM (one-way ANOVA with Tukey’s post-test) **P < 0.01, ***P < 0.001, ****P < 0.0001.