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. 2016 Apr 18;7:516. doi: 10.3389/fmicb.2016.00516

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Copyright © 2016 Bagwell, Abernathy, Barnwell, Milliken, Noble, Dale, Beauchesne and Moeller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ordination plots of elements for cytotoxic Chlorella sp. (strain 15) cultures responding to the addition of new iron. Red lines indicate the relative contribution of sampling time to the positioning of the elements in the ordination. Iron levels were fully restored for Treatment 4 Chlorella cultures (A); i.e., 0.1 mg/L Fe(III)-EDTA and 1.3 mg/L Fe(II)SO₄ × 7H₂O was added to the cultures. Iron-replete conditions were established for the Treatment 5 Chlorella cultures (B); i.e., 40 mg/L Fe(III)-EDTA and 520 mg/L Fe(II)SO₄ × 7H₂O was added to the cultures. Relative cytotoxic activity was inferred by in vivo bioassay and is denoted as highly active (+++), low level of activity (+), and no activity detected (ND).