Table 1.
Cytotoxicity demonstrated for freshwater green algae in response to nutrient limitation.
| Strain | Revised medium formulation | Elutropic solvent series |
||
|---|---|---|---|---|
| DCM | MeOH | H20 | ||
| Scenedesmus sp. (Strain 18B) | Treatment 1 (1/2 PO4, 1000x Cu2+) | – | – | – |
| Treatment 2 (N8: Limited N, Mg, S, Fe) | – | +N2A | – | |
| Treatment 3 (1/100 NO3) | – | – | – | |
| Treatment 4 (1/10 total iron) | +GH4C1/N2A | ++GH4C1/N2A | ++GH4/N2A | |
| Treatment 5 (1/100 total iron) | ND | ND | ND | |
| Chlorella sp. (Strain 15) | Treatment 1 (1/2 PO4, 1000x Cu2+) | – | – | – |
| Treatment 2 (N8: Limited N, Mg, S, Fe) | – | – | – | |
| Treatment 3 (1/100 NO3) | – | – | – | |
| Treatment 4 (1/10 total iron) | – | +++GH4C1/N2A | – | |
| Treatment 5 (1/100 total iron) | – | +++GH4C1/N2A | – | |
The potency of algal cell extracts to the mammalian cells was inferred from the bioassay volume resulting in cell death; thus, 1 μl = +++, 2 μl = ++, and 4 μl = +. Cytotoxic activity is inferred from the immortalized mammalian cell lines GH4C1 and N2A which exhibit predominantly Ca2+ channel activity and Na+ channel activity, respectively. Bioassay results where no cytotoxicity was detected is indicated by ‘–’ and ‘ND’ indicates bioassays could not be performed because of poor growth and insufficient biomass.