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. 2016 Apr 18;6:24168. doi: 10.1038/srep24168

Figure 4. Early dysregulation of STAT3-AMPKα-SOCS3 signaling in lungs of mice with early postnatal hyperalimentation (pHA) and early-onset obesity at postnatal day 21 (P21).

Figure 4

(A) Immunoblots showing lung protein expression of total STAT3, and phosphorylation of STAT3 (pSTAT3) at P21; Ctrl: n = 5 from 4 litters; pHAmouse: n = 5 from 4 litters. (B) Assessment of suppressor of cytokine signaling 3 (SOCS3), a leptin/IL-6 target, by immunoblot at P21; Ctrl: n = 5 from 4 litters; pHAmouse: n = 5 from 4 litters. (C) Representative immunoblot for total AMPKα and phosphorylated AMPKα (p AMPKα) in lungs at P21; Ctrl: n = 6 from 4 litters; pHAmouse: n = 8 from 4 litters. β-ACTIN served as loading Control (Ctrl). The densitometric analyses are presented next to the respective immunoblot. pHAmousegroup: white bar; Ctrl: black bar. Mean ± SEM; Mann Whitney test; *p < 0.05.