Table 1. Comparative Antibacterial Activity and Inhibition of Translation in Eukaryotic and Prokaryotic Systems^a.

	translation inhibitionb		antibacterial activity MIC (μM)c
compd	IC50Euk (μM)	IC50Pro (μM)	E. coli R477/100	B. subtilis ATCC6633
Genta.	62 ± 9	0.03 ± 0.00	6	<0.75
G418	2.0 ± 0.3	0.01 ± 0.00	9	<1.25
1	347.1 ± 34.3	6.0 ± 1.0
(R)-4	120.5 ± 14.5	11.0 ± 1.2	>311	311
(S)-5	515.8 ± 15	91.9 ± 8.4	>375	>375
2	13.9 ± 1.2	1.0 ± 0.1	680	42
6	7.5 ± 0.5	0.7 ± 0.1	>273	34
3	1.5 ± 0.1	1.1 ± 0.2	1267	156
7	1.2 ± 0.1	1.2 ± 0.1	>257	64

^aIn all biological tests, all AGs were in their sulfate salt forms, and the concentrations refer to the free amine form of each AG.

^bThe eukaryotic and prokaryotic half-maximal-inhibition values (IC₅₀^Euk and IC₅₀) were quantified in coupled transcription/translation assays by using active luciferase detection as previously described by us.⁵

^cMinimal inhibitory concentration (MIC) values were determined by using the double-microdilution method.