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. 2013 Jun 27;12:584–597.

Figure 4. Up-regulated expression levels of RUNX2 by BMP-2 promoted BMSC osteogenic differentiation in normal (5.5 mM) and high-glucose (25 mM) conditions.

Figure 4

A: The level of endogenic BMP-2 in BMSCs grown in basal osteogenic medium containing normal (5.5 mM) or high glucose (25 mM), or in the absence or presence of BMP-2 (100 ng/mL) for 7 d were measured by Western blot.

B: Real-time PCR analysis of RUNX2, ALP, and OCN expression in BMSCs after 7-d culture in basal medium (Undiff) and osteogenic medium (Diff), containing normal (5.5 mM) or high glucose (25 mM) in the absence or presence of 100 ng/mL BMP-2. β-Actin was used as a control for equal loading. Results represent the mean ± SD from three independent experiments performed in triplicate. *P < 0.05 vs. the normal /Diff groups