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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Nat Struct Mol Biol. 2016 Feb 1;23(3):225–230. doi: 10.1038/nsmb.3167

Figure 2. U1-70K enhances U1 Sm core assembly, inhibits other snRNAs’ Sm core assembly in vitro, and regulates snRNP repertoire in cells.

Figure 2

(a) Quantitative in vitro Sm core assembly activities on the indicated snRNAs in extracts from cells with control, U1-70K, SMN, Gemin2 or Gemin5 siRNA knockdowns. The Sm core assembly activities on each snRNA were compared to control RNAi extracts (100% activity), except U1ΔSm and U4ΔSm, whose relative activities were compared to pre-U1 and U4, respectively. The error bars represent standard deviation from three independent biological replicates. (b) Quantitative measurements of snRNAs from the mature and nascent snRNPs in cells with control, U1-70K, SMN or Gemin5 knockdown by real-time RT-PCR. The relative amount of snRNAs compared to control RNAi are shown. The error bars represent standard deviation from three independent biological replicates.