Figure 5. Comparison analysis of gene expression between primary mouse Schwann cell cultures and developing sciatic nerve samples.

(A, B) Analysis of the whole-genome revealed distinct gene transcription between in vivo and in vitro, illustrated by PCA (A). A well-defined cluster can be depicted of developing nerve samples, whereas P60 form an individual cluster. Distinct expression between primary Schwann cell cultures and in vivo samples could be confirmed by a heat map analysis, indicated by the dendrogram (B). (C, D) Analysis based on only forskolin-dependent differentially expressed transcripts resulted in distinct clusters between treated and untreated Schwann cells, as well as between in vivo and in vitro (C). Heat map analysis revealed that forskolin-treated Schwann cells associate within the same branch as the samples derived from the nerve tissues (D, arrow).