Figure 1.

Schematic representation of the FZD₄ C-tail variants investigated in this study. The constructs were generated using a template containing a V5 tag inserted in the amino- terminus of the protein following the signal peptide. The residues of the highly conserved KTXXXW domain are colored red. The alanine residues substituted for the lysine and cysteine residues in the mFZD₄KC506-507AA receptor are colored blue. The mFZD₄/C-tailmFZD₁, mFZD₄/C-tailmFZD₃, and mFZD₄/C-tailmFZD₇ constructs differ from the mFZD₄ WT construct. In these chimera, the region of the C-tail distal to the KTXXXW domain of mFZD₄ has been substituted by the corresponding region of mFZD₁ (mFZD₁ residues R626 to V642), mFZD₃ (mFZD₃ residues A508 to A666 of mFZD₃) or mFZD₇ (mFZD₇ residues R556 to V572), respectively. Note that for simplicity the mFZD₄/mFZD₃ C-tail is not shown in its entirety.