Figure 4.

FIV-βgal transduction of lower airway epithelia 5 days after gene transfer. (a) En face view of pleural surface of lung after fixation and X-gal staining showing βgalactosidase–expressing cells. All treated animals had similar segments of β-galactosidase–expressing cells extending to the pleural surface. (b–f) Higher magnification views of tissue sections showing lower airway and parenchymal cells transduced. (b) Low-magnification view of a large bronchus (>750 μm diameter) demonstrating patches of β-galactosidase–expressing cells extending around the circumference of the epithelium. (c) High-magnification view of expression in a large bronchus (>750 μm diameter) showing expression in ciliated cells and basal cells. (d) High-magnification view of expression in a medium sized airway (500–750 μm diameter) demonstrating expression in nonciliated surface cells (Clara cells). (e) β-galactosidase expression in a small bronchus (250–500 μm diameter) showing expression in nonciliated surface cells (Clara cells). (f) Distal lung sample (airways 0–250 μm diameter) showing expression in cuboidal cells consistent with alveolar type II cells. (g) Gene transfer expressed as a function of airway size. Numbers above each bar represent the number of animals with transduced cells in the corresponding region. Tissues from 12 animals were studied. Scale bar in d also applies to e and f.