Figure 4.

Lhx2cKO derived corneal epithelial cells showed delayed wound healing.

Corneal epithelial cells were isolated from WT and Lhx2cKO mice and cultured as indicated in Material and Methods. Cells were seeded in culture plates and when confluent a scratch was made through the cell monolayer and the wound closure followed via time‐lapse microscopy (A). Scratches in WT cells closed in less than 24 hrs, while closure of scratches made in Lhx2cKO cultures were significantly delayed, healing by 48hrs (B). Data are means SE ± (n = 3). *P ≤ 0.05. Abbreviation: WT, wild type.