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. 2016 Apr 18;47:50. doi: 10.1186/s13567-016-0334-0

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© Yi et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SDS-PAGE and Western immunoblotting analysis of the rGroEL protein expressed in E. coli BL21. Lane M, protein molecular mass marker; lane 1, Western blot analysis of puried rGroEL protein using pre-immune mini-pig sera as the negative control; lane 2, Western blot analysis of puried rGroEL protein using mini-pig convalescent sera; lane 3, the elution of the purified rGroEL were separated on an SDS-PAGE and stained with coomassie; lane 4, rGroEL protein in E. coli BL21induced with 1 mM IPTG for 4 h; lane 5, rGroEL protein without IPTG; lane 6, empty expression vector pET28a (+).