Figure 5.

Quantitative microtiter plate assay for biofilm production by S. equi spp. zooepidemicus. Biofilm formation was evaluated by monitoring the A_{595 nm} following crystal violet staining of bacterial cultures. The columns represent the means and standard deviations of three experiments. A Biofilm formation in THB medium (control); B Biofilm formation in THB medium supplemented with normal mouse serum; C Biofilm formation in THB medium supplemented with mouse rGroEL-antiserum; D THB medium. Student’s t-test was performed for the statistical significance analysis. Biofilm formation in THB medium supplemented with anti-rGroEL serum (C) was significantly lower (P < 0.01) than that of S. equi spp. zooepidemicus cultured in THB medium alone (A) or in THB medium supplemented with non-immunized pathogen-free mouse serum (B). No significant differences were found between S. equi spp. zooepidemicus cultured in THB medium alone and S. equi spp. zooepidemicus cultured in the THB medium supplemented with non-immunized mouse serum (P > 0.05).