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. 2016 Feb 15;1(1-2):32–44. doi: 10.1016/j.jacbts.2016.01.009

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Short-Term Histological Assessment of Skeletal Muscle Progenitor Cells

(A) Representative image of Pax-7⁺(red) and Hoechst 33342 (blue) costaining in skeletal muscle matrix (SKM)–injected gracilis muscle. Scale bar is 50 μm and Pax-7⁺ nuclei are indicated by white arrows. There were significantly more Pax-7⁺ cells as a percentage of total nuclei in SKM (n = 6) versus saline (n = 6) injected muscles at (B) day 3, but this returned to quiescent levels by (C) day 10. *p = 0.0096. Data from the healthy limb is shown as a reference.