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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Clin Cancer Res. 2016 Apr 15;22(8):2083–2095. doi: 10.1158/1078-0432.CCR-15-2208

Figure 3. Tumorigenic properties of bulk and CD133-fractionated Accx11 cells.

Figure 3

(A) Cultured bulk Accx11 subcutaneously injected in athymic nude mice produce tumors with histology similar to patient tumor and parental xenograft (H&E staining). (B) CD133, SOX10, NOTCH1, and FABP7 expression confirms ACC identity of PDX and tumors generated from injection of bulk Accx11 cells. (C) Immunoblot analysis confirms similar expression of SOX10, NOTCH1, and FABP7 in PDX and tumor formed from injected bulk Accx11 cells. GAPDH serves as a loading control. (D) Dot plot of tumor volumes 90 days after injection of 105 CD133- or CD133+ MACS-sorted Accx11 cells.