Figure 5.

Permissive histone H3 methylation patterns associate with transcription of T_h1- and T_h17-related factors in γδ T cell subsets. (a) ChIP-seq analysis of H3K4me2 (green) and H3K27me3 (black) modifications on genes encoding T_h1-related factors (Tbx21, Eomes, Hlx; left) and T_h17-related factors (Rorc, Batf, Blk; right) in peripheral γδ27⁺ and γδ27⁻ T cells (below plots, as in Fig 4a). (b) Quantification (log₁₀-transformed) of gene-specific H3K4me2 modifications on genes in γδ27⁺ T cells and γδ27⁻ T cells for genes grouped as T_h1- and T_h17- related factors (top left), genes linked to γδ T cell development (top right), genes associated with alternative effector cell types (bottom left) and housekeeping reference or survival genes (bottom right). (c) ChIP-qPCR analysis of H3K36me3 modifications on the transcription factor–encoding genes Tbx21, Eomes, and Rorc in γδ27⁺ and γδ27⁻ T cells, presented relative to the abundance of total H3. (d) Quantitative RT-PCR analysis of genes encoding T_h1-related factors (top) and T_h17-related factors (bottom) on ex vivo CD4⁺ T cells and γδ27⁺ and γδ27⁻ T cells (derived from peripheral T cells) and CD4⁺ T_h1 and T_h17 cells generated in vitro, presented relative to Actb expression. NS, non significant; *P < 0.05 (Mann-Whitney two-tailed test). (e) Expression of T_h1- and T_h17-associated genes (from data in d) in γδ27⁺ T cells versus γδ27⁻ T cells (top) or CD4⁺ T_h1 cells versus T_h17 cells (bottom). Each symbol (d,e) represents an individual experiment. Data are pooled from five independent experiments with cells pooled from four mice in each (mean and s.d. in c).