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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Nat Methods. 2016 Mar 7;13(4):329–332. doi: 10.1038/nmeth.3800

Figure 2.

Figure 2

Assessment of clonal CD4+ T cell expansion during Salmonella typhimurium infection. (a) Schematic of timeline for Salmonella infection experiment. (b) Distribution of expanded clonotypes within splenic CD4+ T cell populations analysed by single-cell RNA-seq. The x-axis indicates the number of cells within the expanded clonotypes whilst the y-axis represents the number of clonotypes of each size. (c) Clonotype network graph from day 14, mouse 1. Each node in the graph represents an individual splenic CD4+ T lymphocyte. Coloured bars within the nodes indicate the presence of reconstructed TCR sequences that were detected for each cell. Dark coloured identifiers are productive, light coloured are non-productive. Red edges between the nodes indicate shared TCRα sequences whilst blue edges indicate shared TCRβ sequences. Edge thickness is proportional to the number of shared sequences.