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. Author manuscript; available in PMC: 2016 Apr 18.
Published in final edited form as: Cell Rep. 2015 Apr 2;11(1):13–21. doi: 10.1016/j.celrep.2015.03.014

Figure 1.

Figure 1

Three strategies for assigning ribosome occupancy. A) Average ribosome occupancy aligned at the start codon. Reads from the Δefp3 dataset were assigned using the center-assignment strategy (grey) or the 5′ or 3′-ends of the reads (black and blue, respectively). B) Average ribosome occupancy aligned at the stop codon. C) Expanded view at the start codon using the 3′-assignment method. D) Average density aligned at start codons using the 5′-assignment strategy. Each plot represents reads of a single length; eleven plots are shown in different colors for 25 – 35 nt reads. E) Comparison of the three assignment strategies at the stalling motif in secM, where the ribosome stalls with the Gly codon in the P site.