Fig 3. Activity of RNase P-G and RNase P-U holoenzyme complexes at different ammonium acetate concentrations.

The pre-tRNA processing activity of RNase P-G and RNase P-U proteins and their holoenzymes was assayed in the presence of different concentrations of ammonium acetate. To reconstitute the RNase P holoenzyme, 50 nM RNase P RNA and 100 nM RNase P protein were used. For protein alone activity, 100 nM protein was used in the assays.