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. 2016 Apr 18;11(4):e0153579. doi: 10.1371/journal.pone.0153579

Fig 6. Displayed H1 HA stalk peptides elicit antibodies that bind to the native H1 HA protein but do not strongly compete with anti-stalk mAb KB2.

Fig 6

(A) Individual post-vaccination sera were assayed for their reactivity with a recombinant trimeric soluble HA from pandemic CAL09 (H1N1) and (B) were further tested for competition binding to chimeric cH6/1 HA in the presence of 10 μg/mL KB2. Sera were from mice vaccinated with the H3 subtype HIR05 HA (black) or with stalk peptides from a H1 subtype (NC99) virus engineered into its globular head: HIR05/NC99-Ep66-69 (green), HIR05/NC99-Ep86-89 (pink), HIR05/NC99-Ep69+73 (blue) or HIR05/NC99-Ep73+96 (yellow) or from mice vaccinated with the TIV Agriflu® (grey). Due to the lack of enough serum from two mice, there are only three measurements given for vaccine group HIR05/NC99-Ep66-69. * indicates a P value of ≤0.05.