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. 2016 Apr 18;11(4):e0153988. doi: 10.1371/journal.pone.0153988

Fig 4. Extracellular localization of the GbSBT1 protein in tobacco leaves determined through confocal microscopy.

Fig 4

(A) Localization of GbSBT1-YFP in tobacco leaves; localization of GbSBT1 (without signal peptide)-YFP in tobacco leaves; localization of GbSBT1-YFP in tobacco leaves under JA treatment; localization of GbSBT1-YFP in tobacco leaves under ethylene treatment. Arrows indicate the difference in protein localization between normal and phytohormone treatments; (B) GbSBT1 protein co-localized with plasma membrane integral protein PIP1 on the cell membrane using protoplast and tobacco lead epidermal cells as the protein expressing materials. Upper panels: protoplast; lower panels: tobacco leaf epidermal cells.