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. 2016 Apr 18;11(4):e0153630. doi: 10.1371/journal.pone.0153630

Fig 7. Cytokine release from total splenocytes stimulated with OVA.

Fig 7

After the final OVA nasal challenge, the mice were sacrificed, and splenocytes were collected (SSV mAb (n = 4) or isotype IgG1 (n = 5)). Splenocytes were stimulated with OVA for 72 hrs, and culture media were obtained. Cytokine levels (A: IFN-γ, B: IL-4, C: IL-5, D: IL-10, E: IL-13, and F: IL-17) were determined by using specific ELISA kits. Each symbol indicates an individual mouse, and bars show the mean values. NS: not significant.