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. 2016 Apr 18;11(4):e0153825. doi: 10.1371/journal.pone.0153825

Table 1. Summary of the viscum and TT extract batches.

batch plant material viscum extract [mg mistletoe / mL extract] buffer 2-HP-β-CD [mg/mL] OA [mg/mL] ML [μg/mL]
viscum 155 One-year-old shoots of mistletoe from apple trees harvest 03.06.2009 3 mg/g 30 mM phosphate pH 7.6 with ascorbic acid and sucrose - - 1.17 ± 0.17
TT 155 Mistletoe dry extract harvest 09.05.2011 - 60 mM phosphate pH 7.6 with sucrose 250 3.31 ± 0.08 -
viscum 157L One-year-old shoots of mistletoe from apple trees harvest 03.06.2009 4 mg/g 30 mM phosphate pH 7.5 with ascorbic acid - - 1.67 ± 0.32
TT 157L Mistletoe dry extract harvest 09.05.2011 - 60 mM phosphate pH 7.5 230 2.90 ± 0.09 -
viscum 161 One-year-old shoots of mistletoe from apple trees harvest 03.07.2009 3 mg/g 30 mM phosphate pH 7.5 with ascorbic acid and sucrose - - 2.19 ± 0.11
TT 161 Mistletoe dry extract harvest 23.05.2013 - 60 mM phosphate pH 7.5 with sucrose 240 3.60 ± 0.06 -

ML (mistletoe lectin I) was quantified using ELISA (n ≥ 3). OA (oleanolic acid) was quantified in each solution using GC-FID and external calibration with OA as reference substance (n ≥ 3) [45]. Values are mean ± SD.