Table 1. Summary of the viscum and TT extract batches.
batch | plant material | viscum extract [mg mistletoe / mL extract] | buffer | 2-HP-β-CD [mg/mL] | OA [mg/mL] | ML [μg/mL] |
---|---|---|---|---|---|---|
viscum 155 | One-year-old shoots of mistletoe from apple trees harvest 03.06.2009 | 3 mg/g | 30 mM phosphate pH 7.6 with ascorbic acid and sucrose | - | - | 1.17 ± 0.17 |
TT 155 | Mistletoe dry extract harvest 09.05.2011 | - | 60 mM phosphate pH 7.6 with sucrose | 250 | 3.31 ± 0.08 | - |
viscum 157L | One-year-old shoots of mistletoe from apple trees harvest 03.06.2009 | 4 mg/g | 30 mM phosphate pH 7.5 with ascorbic acid | - | - | 1.67 ± 0.32 |
TT 157L | Mistletoe dry extract harvest 09.05.2011 | - | 60 mM phosphate pH 7.5 | 230 | 2.90 ± 0.09 | - |
viscum 161 | One-year-old shoots of mistletoe from apple trees harvest 03.07.2009 | 3 mg/g | 30 mM phosphate pH 7.5 with ascorbic acid and sucrose | - | - | 2.19 ± 0.11 |
TT 161 | Mistletoe dry extract harvest 23.05.2013 | - | 60 mM phosphate pH 7.5 with sucrose | 240 | 3.60 ± 0.06 | - |
ML (mistletoe lectin I) was quantified using ELISA (n ≥ 3). OA (oleanolic acid) was quantified in each solution using GC-FID and external calibration with OA as reference substance (n ≥ 3) [45]. Values are mean ± SD.