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. 2015 Dec 4;11(12):2247–2258. doi: 10.1080/15548627.2015.1112484

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Figure 1. — Elevated expression of LC3-II in different tissues of gadd45a^−/− mice. (A) Western blot analysis of LC3-II levels in liver, lung and kidney tissues of mice. All tested tissues homogenates were loaded and analyzed by 15% SDS-PAGE. (B, F) The densities of signals were determined by densitometry and are shown relative to the Gadd45a^+/+ group. Graphical data denote mean ± SD. (C) Confocal microscopy images and immunofluorescence analysis with anti-LC3 antibody. Scale bar: 10 μm. (D, H) The number of LC3 puncta per cell. Graphical data denote mean ± SD. (E) SQSTM1, LC3 and GADD45A expression variation were analyzed using western blots in MEF Gadd45a^+/+ and gadd45a^−/− cells. (G) MEF Gadd45a^+/+, gadd45a^−/− cells were seeded onto the cover slips and analyzed by immunofluorescence with anti-LC3 antibody. Scale bar: 10 μm. ***,P < 0.001; **,P < 0.01.