Figure 4.

Effect of nutlin-3 treatment on autophagy induction in EBV-negative and EBV-positive latency III lymphoid cell lines. (A) Cells preincubated (30 min) with or without BAF (100 nM) were treated with nutlin-3 for various times. Expression of LC3-I and LC3-II was tested by western blotting of whole cell lysates. (B and C) Cells treated with nutlin-3 for 16 h were stained with MDC. The percentages of cells (means from 3 independent experiments) with MDC stained dots were assessed by fluorescence microscopy (B). The MFI of MDC stained dots was assessed by flow cytometry (C). (D) Cells incubated with or without nutlin-3 for 16 h were stained with the Cyto-ID Autophagy detection kit. The MFI of cyto-ID staining was assessed by flow cytometry.