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. 2015 Nov 13;11(12):2358–2369. doi: 10.1080/15548627.2015.1106779

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — Autophagy mediated the upregulation of CoPs-induced osteoblast apoptosis in vivo. (A) Immunofluorescence was performed to determine the expression of cleaved CASP3 in osteoblasts. Sections of murine calvaria are presented for the animals from each group. Scale bar: 50 µm. Red, cleaved CASP3 (C-CASP3); green, osteoblasts (BGLAP); blue, DAPI nuclear staining. (B) Immunofluorescence was performed to determine the colocalization of LC3 and cleaved CASP3 in osteoblasts. Sections of murine calvaria are presented for the animals from each group. Scale bar: 50 µm. Red, LC3; green, cleaved CASP3 (C-CASP3); blue, DAPI nuclear staining.