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. 2015 Nov 13;11(12):2172–2183. doi: 10.1080/15548627.2015.1106663

Figure 2.

Figure 2.

The increased NUPR1 and TIA1 levels induced by LPS and oxLDL were inhibited by 3BDO. (A) HUVECs were exposed to 50 μg/ml nLDL or oxLDL with or without 3BDO (60 μM) for 24 h, then 20 and 40 μg cell extracts was loaded on the SDS-PAGE respectively. Western blot analysis of NUPR1 and TIA1 protein levels, with ACTB and Ponceau staining as the loading control, and (B) quantification. (C) HUVECs were treated with LPS (1 μg/ml, 6 h) with or without 3BDO (60 μM), then 20 and 40 μg cell extracts was loaded on SDS-PAGE respectively. Western blot analysis of TIA1 protein level, with ACTB and Ponceau staining as the loading control, and (D) quantification. *, P < 0.05; **P < 0.01; n = 3.