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. 2015 Nov 13;11(12):2172–2183. doi: 10.1080/15548627.2015.1106663

Figure 3.

Figure 3.

NUPR1 and TIA1 were involved in regulation of TGFB2-OT1 level. qPCR analysis of TGFB2-OT1 mRNA levels in HUVECs subjected to scrambled siRNA (Scr) or NUPR1 siRNA (siNUPR1, 40 nM) for 24 h, then exposed to LPS at various concentrations for 3 h (A) or treated with 1 μg/ml LPS for 3 or 12 h (B). (C) qPCR analysis of TGFB2-OT1 mRNA levels in HUVECs subjected to scrambled siRNA (Scr) or TIA1 siRNA (siTIA1, 40 nM) for 24 h, then treated with 1 μg/ml LPS for 6 h. (D) Western blot analysis of the TIA1 protein level in HUVECs subjected to scrambled siRNA (Scr) or NUPR1 siRNA (siNUPR1, 40 nM) for 24 h, then exposed to 1 μg/ml LPS for 6 h. *, P < 0.05; **, P < 0.01; #, P > 0.05; n = 3.