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. 2015 Nov 13;11(12):2172–2183. doi: 10.1080/15548627.2015.1106663

Figure 4.

Figure 4.

MIR3960 and MIR4488 directly bind to TGFB2-OT1. (A) qPCR analysis of MIR3960 and MIR4488 level in HUVECs treated with or without 3BDO (0, 15, 30 and 60 μM) for 24 h and (B) transfected with the empty vector pCMV6 (0.8 μg/cm2, indicated by 0 in the figure) or pCMV6-TGFB2-OT1 plasmid (0.2, 0.4 and 0.8 μg/cm2) for 24 h. (C) Luciferase activity assay with Luc-TGFB2-OT1-WT and Luc-TGFB2-OT1-3960Δ plasmids cotransfected into HEK293 cells with 80 nM MIR3960 mimics or negative control for 24 h. (D) Luciferase activity assay with Luc-TGFB2-OT1-WT and Luc-TGFB2-OT1-4488Δ plasmids cotransfected into HEK293 cells with 80 nM MIR4488 mimics or negative control for 24 h. *, P < 0.05; **, P < 0.01; n = 3.