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. 2015 Nov 13;11(12):2172–2183. doi: 10.1080/15548627.2015.1106663

Figure 5.

Figure 5.

MIR3960, MIR4488 and MIR4459 targeted CERS1, NAT8L and LARP1. Western blot analysis of protein levels of CERS1 (A) and NAT8L (B) in HUVECs transfected with 25 and 50 nM MIR3960 (A), MIR4488 (B) mimics, inhibitor or negative control for 24 h and quantification. (C) Western blot analysis of protein levels of LARP1 in HEK293 cells (left) and HUVECs (right) transfected with 25 and 50 nM MIR4459 mimics or negative control for 24 h and quantification. (D) Luciferase activity assay with Lu-CERS1-3′UTR, Lu-NAT8L-3′UTR, and Lu-LARP1-3′UTR plasmids cotransfected into HEK293 cells with 50 nM MIR3960, MIR4488 and MIR4459 mimics for 24 h, a negative miRNA as a control. *, P < 0.05; **, P < 0.01; n = 3.