3BDO and TGFB2-OT1 overexpression regulated the protein levels of CERS1, LARP1 and NAT8L. (A) Western blot analysis of protein levels of CERS1, LARP1 and NAT8L in HUVECs treated with 3BDO (0, 15, 30, 60 and 120 μM) for 24 h and (B) quantification. (C) Western blot analysis of protein levels of CERS1, LARP1 and NAT8L in HEK293 cells transfected with the empty vector pCMV6 (0.8 μg/cm2, indicated by 0 in the figure) or pCMV6-TGFB2-OT1 plasmid (0.2, 0.4 and 0.8 μg/cm2) for 48 h and (D) quantification. *, P < 0.05; **, P < 0.01; n = 3.