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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Bone. 2016 Feb 3;85:123–130. doi: 10.1016/j.bone.2016.01.023

Fig. 1. CM from WT BMMs, but not Cox2 KO BMMs, inhibited PTH-stimulated osteoblast differentiation and cAMP production.

Fig. 1

For all experiments, CM was collected from either the WT BMMs (WT CM) or the Cox2 KO BMMs (KO CM) that were stimulated with Rankl for 3 days. (A) Cox2 KO POBs were cultured for 14 days in the presence of no CM (differentiation medium), WT CM, or Cox2 KO CM and either vehicle or PTH (10 nM). On day 14 of culture, Alp mRNA expression was measured by qRT-PCR. (B) On day 5 of culture, Cox2 KO POBs were treated with vehicle or PTH (10 nM) for 3 hours. Runx2 expression was measured by qRT-PCR. (C) On day 5 of culture, Cox2 KO POBs were treated with vehicle or PTH (10 nM) for 15 minutes. cAMP was measured by ELISA. (D) On day 5 of culture, Cox2 KO POBs were treated with vehicle or PTH (10 nM) for 3 hours. c-Fos expression was measured by qRT-PCR. Bars represent mean ± SEM, n=3. aSignificant effect of PTH, p<0.01. bSignificant difference compared to WT CM, p<0.01.