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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Bone. 2016 Feb 3;85:123–130. doi: 10.1016/j.bone.2016.01.023

Fig. 3. PTH phosphorylated β-catenin at ser552 and ser675 in a PKA- dependent manner.

Fig. 3

Cox2 KO POBs were treated with vehicle or 10 nM PTH on day 5 of culture and Western analysis was performed. CM was collected from either the WT BMMs (WT CM) or the Cox2 KO BMMs (KO CM) that were stimulated with Rankl for 3 days. (A) Cultures treated with 10 nM PTH for 15 minutes following WT/KO CM and ± H89 (30 μM) pretreatment. (B) Cultures treated as noted with the addition of ± GF109203X (1 μM). (C–G) Densitometry was performed on KO CM treated groups, values were normalized to β-actin and vehicle-treated groups were set to 1. Bars represent means ± SEM, n=3. aSignificant effect of PTH, p<0.05.