Figure 2.

Effector T cells are suppressed by multipotent adult progenitor cell (MAPC)-conditioned medium in a dose-dependent manner in RA and HD. MAPCs were grown in serum-free MAPC medium that contains tumor necrosis factor-α (10 ng/ml), interleukin-1β (10 ng/ml), and an equivalent amount of interferon (IFN) γ. After 3 days IFNγ was immunodepleted. Control growth medium was used as a control for CCM; it is the initial medium but has not been exposed to MAPCs. CCM concentrations between 12.5% and 87.5% of the total medium were tested. Suppression was observed at CCM concentrations of greater than 50% by volume. Abbreviations: CCM, multipotent adult progenitor cell-conditioned medium; HD1–HD3, blood from healthy donors specific to this experiment; RA1–RA4, distinct rheumatoid arthritis patients specific to this experiment.